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KMID : 0545120150250111920
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 11 p.1920 ~ p.1927
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Optimization of Influencing Factors on Biomass Accumulation and 5-Aminolevulinic Acid (ALA) Yield in Rhodobacter sphaeroides Wastewater Treatment
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Liu Shu Li
Li Xiang Kun
Zhang Guang Ming
Zhang Jie
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Abstract
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This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe2+, Mg2+, Ni2+, and Zn2+ further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe2+ addition.
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KEYWORD
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Rhodobacter sphaeroides, 5-aminolevulinic acid, influencing factors, optimization, wastewater treatment
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